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A Handy Method to Estimate Titer of Classical Swine Fever Virus in PK-15 Cells
Volume 9 | Issue 3 | December 2023
AUTHOR(S)
Chayna Singha Mahapatra, Aruna Kuniyal, Shyma K. Latheef, Pronab Dhar

ABSTRACT

Determination of virus titer is the key factor for development and quality control of classical swine fever (CSF) cell culture vaccines. Like any other non-cytopathic effect producing viruses, classical swine fever virus titration is done by immunological methods e.g. Fluorescent Antibody Test (FAT). These classical assays for the determination of Tissue Culture Infective Dose 50 (TCID50) are time-consuming and require huge reagents. Hence, they are not very suitable for handling a large number of samples. An accurate and reliable technique for TCID50 determination by FAT in cover-slip culture was optimized for titration of classical swine fever. Optimization of FAT in LAB TEK slide chamber could do away the tedious manipulation of cover-slip culture without affecting its final outcome. This newly optimized method of titration has shown 100% similar result to that of one full proof titration method of CSF virus in PK-15 cells in terms of fluorescent positive cells in wells and their fluorescent intensity.

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DOI
https://doi.org/10.62418/ijvph.9.3.2023.32-35
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Corresponding author’s email ID: drpdhar@gmail.com
Citation: Mahapatra CS, Kuniyal A, Latheef SK, Dhar P. A Handy Method to Estimate Titer of Classical Swine Fever Virus in PK-15 Cells. Indian Journal of Veterinary Public Health. 2023; 9(3): 32-35.
DOI: https://doi.org/10.62418/ijvph.9.3.2023.32-35