Diagnosis of Brucellosis under Field Conditions Using Gold Nanoparticle-Based Lateral Flow Technology and Compared with Other Serological Tests
Volume 11| Issue 2 | December 2025
AUTHOR(S)
Pradeep A, Sujatha Singh, Vijaya Kumar A, Kalyani P, Krishnaiah N

ABSTRACT

The annual prevalence of brucellosis was determined in the buffalo population residing in and around the Hyderabad district of Telangana, India. A total of 204 vaginal samples of buffaloes collected from private dairy farms, were subjected to PCR assay, ELISA and developed kit on lateral flow technology, to detect Brucella organisms. Out of the total samples screened, 113(55.4%), 92(45.1%) and 87 (42.64%) were found to be positive, respectively. Considering PCR assay as the gold standard method, the laboratory developed kit based on lateral flow technology, has shown 76.99% and 100% sensitivity and specificity, respectively.

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DOI
https://www.doi.org/10.62418/ijvph.11.2.2025.23-31
How to cite this article:
Corresponding author’s email ID: sujathasingh.hem@gmail.com
Citation: A Pradeep, Singh S, A Vijaya Kumar, P Kalyani, N Krishnaiah. Diagnosis of Brucellosis under Field Conditions using Gold Nanoparticle-Based Lateral Flow Technology and Compared with Other Serological Tests. Indian Journal of Veterinary Public Health. 2025; 11(2): 23-31.
DOI: https://www.doi.org/10.62418/ijvph.11.2.2025.23-31