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Characterization of Escherichia coli Isolated from Raw Cow Milk from Shops in Asella Town, South East, Ethiopia
Volume 10 | Issue 2 | December 2024
AUTHOR(S)
Asaminew Getu Ayele, Amana Feyisa Amesa, Leta Muleta Kisi, Tesfaye Rebuma Abdeta, Tamasgen Ragasa, Mahendra Pal

ABSTRACT

Foodborne infections are an important challenge to public health and cause significant economic problems in many countries of the world. This research was conducted to assess the occurrence of Escherichia coli in raw milk samples from retail shops, to estimate effect of practices associated with milking and post-milking processes and to determine the antimicrobial susceptibility profile of E. coli isolates. A cross-sectional study was conducted from November 2018 to April 2019 from raw cow’s milk shops in Asella town. A simple random sampling strategy was followed and a total of 384 raw milk samples were collected and immediately processed for E.coli isolation and identification by using selective media and biochemical tests. From 384 samples, 177(46.09%) were positive for E. coli. Hygienic condition of the milk container, equipment washing practice, and milk shop location were found to be significantly associated with the prevalence of E. coli. Forty two (10.94%) E.coli were from stainless steel and 135 (35.16%) from plastic milk containers from milk shops, with statistically significant differences (𝑃 = 0.001). However, the prevalence of isolated E. coli was 33.33% in poor hygienic sanitation and 12.76% in good hygienic sanitation, with a statistically significant difference (P-value = 0.006). Randomly selected 15 E. coli isolates were subjected to antimicrobial sensitivity test with seven commonly used antimicrobials disks. E. coli isolates showed susceptibility to tetracycline (73.33%) and sulfamethoxazole-trimethoprim (66.66%). and were resistant to kanamycin (100%), penicillin-G (100%), amoxicillin (100%), and erythromycin (86.66%). Hence, attention should be given to proper handling of the raw milk and to use susceptible antibiotics in the treatment of diseases both in humans and animals.

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DOI
https://doi.org/10.62418/ijvph.10.2.2024.58-64
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*Corresponding author’s email ID: palmahendra2@gmail.com
Citation: Ayele AG, Amesa AF, Kisi LM, Abdeta TR, Ragasa T, Pal M. Characterization of Escherichia coli Isolated from Raw Cow Milk from Shops in Asella Town, South East, Ethiopia. Indian Journal of Veterinary Public Health. 2024; 10(2): 58-64.
DOI: https://doi.org/10.62418/ijvph.10.2.2024.58-64