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Antibiogram Assay of Listeria monocytogenes Isolates from Milk Samples in and Around Kolkata
Volume 9 | Issue 3 | December 2023
AUTHOR(S)
Monmon Saha, Saurabh Majumder, Chanchal Debnath, Amiya Kumar Pramanik, Debasish Murmu

ABSTRACT

Listeria monocytogenes is well-known globally as one of the most significant foodborne bacterial pathogens. Listeriosis may trigger life-threatening illnesses such as severe sepsis and meningitis, sometimes resulting in lifelong harm and even death. This study aimed to determine the occurrence and antibiotic resistance pattern of L. monocytogenes in a milk sample collected from in and around Kolkata. For this, a total of 104 milk samples [from individual cow udders (n = 36) and pooled can milk collected from farms (n = 20) as well as from the market (n = 48)] were examined for a period of 6 months, starting from January 2014 to June 2014. For the isolation of L. monocytogenes, samples were cultured on selective media and tested for their susceptibility to common antibiotics by disk diffusion assay. The results revealed that the overall occurrence of Listeria speciesin unpasteurized raw milk was 14 (13.46%), and L. monocytogenes was 5 (4.81%). All five L. monocytogenes isolates were subjected to an antibiotic sensitivity test using the Kirby-Bauer disc diffusion method. In this method, three antibiotics tetracycline (100%), gentamycin (100%), and penicillin (100%) exhibited complete sensitivity. However, the isolates showed variable resistance against ampicillin (16.21%), vancomycin (21.62%), and penicillin (43.24%).

Altunta EG, Kocan D, Cosansu S, Ayhan K, Juneja, VK, Materon L. Antibiotic and bacteriocin sensitivity of Listeria monocytogenes strains isolated from different foods. Journal of Food and Nutrition Sciences. 2012; 3(3): 363-68.
Anon S. Performance standards for antimicrobial disk susceptibility Test. 6th ed. Approved Standard document. 1997. Wayne, PA: NCCLS.
Bauer AW, Kirby WM, Sherris JC. Antibiotic susceptibility testing by a standard single disc method. American Journal of Clinical Pathology. 1966; 45: 493–96.
Enurah LU, Aboaba OO, Nwachukwu SCU, Nwosuh, CI. Antibiotic-resistant profiles of food (fresh raw milk) and environmental (abattoir effluents) isolates of Listeria monocytogenes from the six zones of Nigeria. African Journal of Microbiology Research. 2013; 7(34): 4373-78.
Farber JM, Peterkin PI. Listeria monocytogenes, a food-borne pathogen. Microbiology.1991; Rev. (55): 476-511.
National Committee for Clinical Laboratory Standards. Performance standards for antimicrobial susceptibility tests, 4th Edition, NCCLS. 1993; 10(7).
Rocourt J, Catimel B. Biochemical characterization of species in the genus Listeria. Zentralble Bakteriologika Mikrobiologi Hygiene. 1985; 260: 221-31.
Sharif J, Nillayat MM, Sheikh GN, Roy SS, Bhat SA. Prevalence and antibiogram of Listeria monocytogenes in case of abortion and Stillbirth in sheep of Kashmir. Journal of Veterinary Public Health. 2010; 9(1): 43-6.
Shu Bing Y, Jing Tao L, Qiang Zhong Z. Isolation of Listeria monocytogenes from food and test of drug susceptibility. Journal of China Tropical Medicine. 2004; 4(4): 515-34.
Singh R. Indian Dairy and Products Annual Report 2010. USDA Foreign Agricultural Service: Global Agricultural Information Network. Retrieved 16 June 2011, from static.globaltrade.net/files/pdf/20110226231255627.pdf.
USDA. 1999. United States Department of Agriculture. Consumer Information from USDA, Food Safety Education Office, FoodSafety and Inspection service, USDA, Washington D.C. 20250-3700.

DOI
https://doi.org/10.62418/ijvph.9.3.2023.42-46
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Corresponding author’s email ID: sauravviro@gmail.com
Citation: Saha M, Majumder S, Debnath C, Pramanik AK, Murmu D. Antibiogram Assay of Listeria monocytogenes isolates from Milk Samples in and around Kolkata. Indian Journal of Veterinary Public Health. 2023; 9(3): 42-46.
DOI: https://doi.org/10.62418/ijvph.9.3.2023.42-46