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Evaluation of Experimental Pathogenicity of Naganishia albida in Mice
Volume 9 | Issue 1 | December 2022
AUTHOR(S)
Nurul Islam, Rupjyoti Bharali, Hridip Kumar Sarma, Sailen Talukdar, Shameem Ara Begum, Afzal Hoque Akand, Subhash Sarma

ABSTRACT

Virulence factors of non-neoformans species of Cryptococcus have rarely been studied. The present investigation was conducted to elucidate the virulence factors of non-neoformans Cryptococcus species, biological characteristics of the fungal pathogen, and pathophysiology of mice infected with the Cryptococcal species – Naganishia albida comparing with normal mice for definite time intervals. In the current study, all the mice were inoculated with the yeast cells of the cryptococcal organism that showed an almost uniform pattern of behavior in relation to the isolation of the same specific organisms from different organs of the infected mice during the interval of the experimental period on the 2nd, 7th, 14th, 21th and on the 28th day of post-infection after processing the tissue samples from the both dead and also from the mice sacrificed on the scheduled day of post-infection except those of the control group. The yeast cells were cultured in the SDA media and biological characteristics-i.e. cultural and bio-chemical studies from the harvested tissues of liver, brain, kidneys, lungs etc. and also with the physiopathology of mice infected with the species of the Cryptococcal organism were observed for virulence factors in mice model. This study suggested that the pathogenicity of non-neoformans species of the genus did not differ substantially from the other strains of Cryptococcus neoformans in respect of mechanisms of infection, invasion, host tissue damage, and also the important role of cell-mediated immunity. The findings of the present study also indicated that the cultural and biochemical characteristics and also pathophysiology of the species Naganishia albida in the mice model may substantially be significant. However, in the study, Naganishia albida could be recovered from the harvested tissues of different organs on the 2nd day of post-infection itself. Most of the isolates of this species kept the virulence factors that appeared before inoculation but the same were not related to time intervals for 2nd to 28th days of post inoculation. This reflects that virulence factors are not related to time intervals in mice infection regiment before organ isolation. As cryptococcosis is a zoonotic disease, study of virulence factors of the organisms in mice modal may help in understanding the transmission of this disease and its pathogenicity.

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DOI
https://doi.org/10.62418/ijvph.9.1.2022.11-23
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Corresponding author email ID: drnurul68@rediffmail.com
Citation: Islam N, Bharali R, Sarma HK, Talukdar S, Begum SA, Akand AH, Sarma S. Evaluation of Experimental Pathogenecity of Naganishia albida in Mice. Indian Journal of Veterinary Public Health. 2022; 9(1): 11-23.
DOI: https://doi.org/10.62418/ijvph.9.1.2022.11-23