IJVPH

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Unveiling the Cultivation Dynamics of Thermophilic Campylobacters in Different Culture Media Substrate
Volume 9 | Issue 2 | June 2023
AUTHOR(S)
Nawal Kishor Singh, Ajay Kumar Upadhyay, Maansi, Aman Kamboj, Ajay Kumar

ABSTRACT

Campylobacter species, known for their potential zoonotic transmission, play a significant role in instigating enteritis across a diverse array of animals, including those in captive, wild, and domestic environments, alongside avian populations. We isolated Campylobacter jejuni and Campylobacter coli strains from faecal specimens sourced from wild animals. The sample collection encompassed 521 faecal samples procured from wildlife sanctuaries, zoos, and national parks situated in Uttar Pradesh, Uttarakhand, and Chhattisgarh. Employing a meticulously aseptic approach throughout the collection and isolation process, the growth patterns of the identified Campylobacter strains were meticulously scrutinized using five distinct artificial culture media, classified into two groups: blood-free and blood-containing media. The prevalence of Campylobacter spp. was observed to be 11.90% (62 out of 521 samples), with Campylobacter jejuni accounting for 7.10% and Campylobacter coli for 4.80% of the isolates. Subsequent enrichment and plating on Columbia Blood Agar (CBA) supplemented with selective additives demonstrated a notably higher prevalence (11.90%) of Campylobacter spp. compared to alternative media such as Modified Charcoal Cefoperazone Deoxycholate Agar [mCCDA] (10.56%) and Sheep Blood Agar [BA] (8.25%). The culture of Campylobacter strains exhibited the lowest isolation rates on Chocolate Agar [CA] (5.76%) and Hi-chrome Campylobacter Agar [HCCA] (4.22%). The findings from multiplex PCR assays confirmed both the precise identification of Campylobacter species and the efficacy of each culture method employed in this study.

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DOI
https://doi.org/10.62418/ijvph.9.2.2023.33-36
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Corresponding author’s email ID: ajay.akup@gmail.com
Citation: Singh NK, Upadhyay AK, Maansi, Kamboj A, Kumar A. Unveiling the Cultivation Dynamics of Thermophilic Campylobacters in Different Culture Media Substrate. Indian Journal of Veterinary Public Health. 2023; 9(2): 33-36.
DOI: https://doi.org/10.62418/ijvph.9.2.2023.33-36