IJVPH

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Studies on the Evaluation of Antibacterial Activities of Aloe barbadensis and Ocimum gratissimum Against Selected Zoonotic Bacterial Strains
Volume 10 | Issue 1 | June 2024
AUTHOR(S)
Motuma Regassa, Wubit Tafese, Tesfaye Rebuma, Mekonnen Addis, Mahendra Pal

ABSTRACT

Antimicrobial resistance poses a serious public health problem throughout the world, Traditional remedies, of which 95% are derived from plants, are used in Ethiopia to cure 90% of cattle and over 80% of human patients. Traditional medicine continues to be the predominant form of healthcare in underdeveloped nations. Aloe barbadensis and O. gratissimum are used to cure a variety of sick conditions, both infectious and not. The emergence of antimicrobial resistance (AMR) in bacteria is a significant risk and issue associated with conventional antibiotic treatment. Therefore, disc diffusion and minimum inhibitory concentration (MIC) experiments against certain zoonotic pathogenic bacteria were used to assess the antibacterial activity of leaf extracts from chosen plants in vitro.The extraction solvents employed in this investigation were ethanol, methanol, and aqueous extraction. According to the results of the antimicrobial experiment, the zones of inhibition created by the disc diffusion method ranged in size from 2±0.26 mm lower to 30±0.34 mm highest for the three extraction techniques, with methanol extraction producing the highest value of 30±0.34 mm. The ethanol extract from dried leaves of O. gratissimum and A. barbadensis was 10±0.22 mm, lowest to 26±0.34 mm, highest, and 7±0.11mm to 24±0.26 mm, respectively. The zones of inhibition for dried O. gratissimum methanol extract were 15±0.26mm lowest to 30±0.34mm highest and for A. barbadensis, 15±0.26 to 28±0.11. O. gratissimum dried leaves with water extract showed 4±0.37 mm lowest to 10±0.26 mm highest and 6±0.26 mm to 12±0.22 mm, respectively, in both hot and cold conditions. Aloe barbadensis dried leaves showed 2±0.11 to 10±0.29 for hot conditions and 3±0.11 lowest to 12±0.26 highest for cold conditions. For each examined microorganism, there was a significant difference (P<0.05) in ethanol, methanol, and hot and cold aqueous extracts. The findings of this study point to the potential use of A. barbadensis and O. grattissimum extracts as antibacterial agents for developing new pharmaceuticals to control animal pathogenic bacteria responsible for severe sickness.

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DOI
https://doi.org/10.62418/ijvph.10.1.2024.43-53
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Corresponding author’s email ID: palmahendra2@gmail.com
Citation: Regassa M, Tafese W, Rebuma T, Addis M, Pal M. Studies on the Evaluation of Antibacterial Activities of Aloe barbadensis and Ocimumgratissimum against Selected Zoonotic Bacterial Strains. Indian Journal of Veterinary Public Health. 2024; 10(1): 42-53.
DOI: https://doi.org/10.62418/ijvph.10.1.2024.43-53