Molecular Characterization and Antibiotic Resistance Patterns of ESBL-producing Escherichia coli Strains from Bovine Faecal Samples in West Bengal
Volume 9 | Issue 3 | December 2023
AUTHOR(S)
Zahid H. Hazarika, Samir Dey, Siddhartha Narayan Joardar, Indranil Samanta, Sharmila Latchubhukta, Tousif Mondal, Kunal Batabyal

ABSTRACT

Escherichia coli is mainly a commensal and can cause intestinal and urinary tract infections in bovines at times. Mastitis is also one of the E. coli complications. E. coli is one of the enteric pathogens that remain associated with bovine diarrhoea. These commensals may also possess different antimicrobial resistance genes which can make their treatment quite difficult making the bovine diarrhoea cases more serious. The study was aimed to detect the E. coli isolates from bovine faecal samples followed by their characterization, detection oftheir ESBL and ACBL-producing genes, followed by antibiogram. A total of 46 E. coli strains (45.09%) were isolated from bovine faecal samples (n=102) from different districts of West Bengal. All the isolates showed typical characteristics and were found to possess a 16S rRNA gene specific to this genus. During the detection of ESBL and ACBL positivity, 21 (45.6%) and 37 (80.4%) strains showed phenotypical characteristics. A total of 24 (52.17%) and 41 (89.13%) isolates showed the presence of any one of the beta-lactamases (ESBL and ACBL) producing genes in genotypical characterization. All the ESBL-producing E. coli isolates showed a high level of resistance to amoxicillin/clavulanic acid, ceftriaxone, ceftazidime, ampicillin/cloxacillin, cefotaxime (all 100%), levofloxacin (95.83%), cefoxitin (91.67%) and tetracycline (87.5%), whereas gentamicin, imipenem, amikacin (all 100%) and doxycycline (91.67%) were found to be sensitive against these pathogens.

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DOI
https://doi.org/10.62418/ijvph.9.3.2023.59-63
How to cite this article:
Corresponding author’s email ID: drkb.micro@gmail.com
Citation: Hazarika ZH, Dey S, Joardar SN, Samanta I, Latchubhukta S, Mondal T, Batabyal K. Molecular Characterization and Antibiotic Resistance Patterns of ESBL-producing Escherichia coli Strains from Bovine Faecal Samples in West Bengal. Indian Journal of Veterinary Public Health. 2023; 9(3): 59-63.
DOI: https://doi.org/10.62418/ijvph.9.3.2023.59-63