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A Cross Sectional Study on Use of Antibiotic Growth Promoters and Status of Antimicrobial Resistance in Intestinal Non-fastidious Aerobic Bacterial Microflora of Broilers in Vindhya Region of Madhya Pradesh
Volume 11| Issue 2 | December 2025
AUTHOR(S)
Baleshwari Dixit, Ajit Pratap Singh, Serlen Tomar, Ripan Biswas, RV Singh, Manu Dixit, Amit K Jha

ABSTRACT

The usage of antibiotic growth promoters (AGPs) in poultry farming is debatable and use is regulated in some countries including India as their use has raised the concern of positive selection of antimicrobial resistance (AMR) genes in bacteria. With this concern a cross sectional study was carried out to know the uses of AGP among broiler in Vindhya region of Madhya Pradesh by questionnaire survey and identify the antimicrobial resistance (AMR) in four non-fastidious aerobic bacteria of intestine in broilers. A total of 100 broiler farms were surveyed and 300 intestinal samples were collected and processed for isolation and identification of Staphylococcus, Pseudomonas, E. coli and Salmonella spp. by cultural and biochemical methods and antibiotic susceptibility test. Statistical analysis was carried out by log linear analysis to detect the significance of association between these categorical variables. On questionnaire survey 54% (54/100) responded about usage of antibiotics in their farm, 42 % did not respond and remaining 4% did not use any antimicrobials. One of the five antibiotics including enrofloxacin, cephalexin, levofloxacin, tylosin and azithromycin is being used by the farmers irregularly in feed or drinking water. Antibiotic sensitivity test indicated that all the isolated bacteria were multi drug resistant and a variable resistance and senstivity was detected for the antimicrobials of critical clinical importance in human and animals. Antibiotic sensitivity pattern for the antibiotics being used by the farmers of study region showed highest senstivity to levofloxacin and highest resistance to tylosin and azithromycin among the isolated bacteria.

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DOI
https://www.doi.org/10.62418/ijvph.11.2.2025.32-38
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Corresponding author’s email ID: baleshwarivpe@gmail.com
Citation: Dixit B, Singh AP, Tomar S, Biswas R, Singh R, Dixit M, Jha AM. A Cross Sectional Study on Use of Antibiotic Growth Promoters and Status of Antimicrobial Resistance in Intestinal Non- fastidious Aerobic Bacterial Microflora of Broilers in Vindhya Region of Madhya Pradesh. 2025; 11(2): 32-38.
DOI: https://www.doi.org/10.62418/ijvph.11.2.2025.32-38