IJVPH

  • Print ISSN: 0976-948X
  • Online ISSN: 3048-5738
Identification of Transposable Elements Responsible For AMR in Food Borne Pathogen
Volume 9 | Issue 2 | June 2023
AUTHOR(S)
Priyanka K Thakur, Ranjeet Parihar, Seema Rani Pegu, Rajib Deb, Jagana Niharika, Pranab Jyoti Das, Gyanendra Singh Sengar, Joyshik Sonowal, Vivek Kumar Gupta

ABSTRACT

Despite the fact that the presence of silent genes, Transposable elements (TEs) and antibiotic resistance are related, only a small number of studies have examined the presence and distribution of transposable elements in animal food production chains, including pig and poultry farms. It is well known that there is a correlation between the spread of TEs carrying harmful bacteria from farms to food animals and from food animals into the cycle of production of animals for food. The present study identified the presence of five TEs namely Tn6763 (Accession number: OQ565300), Tn6764, (Accession number: OQ565299), Tn6765 (Accession number: OQ409902), Tn2003 (Accession number: OQ503494) and Tn6020 (Accession number: OQ503493) in (N= 235) samples collected from piggery farm and chicken meat shops from Kolkata and some regions of Assam. The identified food borne pathogens mainly belongs to Enterobacteriaceae family. Four highly zoonotic food borne pathogens with AMR was identified, 42% (77) Salmonella enterica, 26% (49) E. coli, 14% (26) Proteus mirabilis, 12% (22) Klebsiella pneumoniae and 6% (11) K. pneumonia were positive and carrying Tn6765, Tn6764, Tn6763, Tn6020 and Tn2003 respectively. Given the presence of TEs responsible for AMR in food borne pathogen and their relative contamination from pig farms and poultry farm to meat shops was described to be a major public health threat. Thus, preventative measures are vital for avoiding the spread of mobile genetic resistance determinants in the Livestock and Animal food production sector and to monitor their emergence.

Amirianshoja T, Junin R, Idris AK, Rahmani O. A comparative study of surfactant adsorption by clay minerals. Journal of Petroleum Science andEngineering. 2013; 101: 21-7.
Babakhani S, Oloomi M. Transposons: the agents of antibiotic resistance in bacteria. Journal of basic microbiology. 2018; 58(11): 905-17.
Bakhshi B, Fallahzad S, Pourshafie M. The occurrence of atypical enteropathogenic Escherichia coli strains among children with diarrhea in Iran. Journal of Infection and Chemotherapy 2013; 19(4): 615-20.
Beutlich J, Jahn S, Malorny B, Hauser E, Hühn S, Schroeter A, Guerra B. Antimicrobial resistance and virulence determinants in European Salmonellagenomic island 1-positive Salmonella enterica isolates from different origins. Appliedand Environmental Microbiology. 2011; 77(16): 5655-64.
Bhat A,Ghatage T, Bhan S, Lahane GP, Dhar A, Kumar R, Pandita T K. Role of Transposable Elements in Genome Stability: Implications for Health andDisease. International journal of molecular sciences. 2022; 23(14): 7802.
Chen YP, Lei CW, Kong LH, Zeng JX, Zhang XZ, Liu BH,Wang HN. Tn 6450, a novel multidrug resistance transposon characterized in a Proteus mirabilis isolate from chicken in China. Antimicrobial agents and chemotherapy. 2018; 62(4): e02192-17.
Colonna Romano N,FantiL. Transposable elements: major players in shaping genomic and evolutionary patterns. Cells. 2022; 11(6): 1048.
Dong P, Wang H, Fang T, Wang Y, Ye Q. Assessment of extracellular antibiotic resistance genes (eARGs) in typical environmental samples and the transforming ability of eARG. Environment international. 2019; 125: 90-6.
Florindo TJ, Florindo GDM, Talamini E, Da Costa JS, De Léis CM, Tang WZ, Ruviaro CF. Application of the multiple criteria decision-making (MCDM) approach in the identification of Carbon Footprint reduction actions in the Brazilian beef production chain. Journal of Cleaner Production. 2018; 196: 1379-89.
Galiot L, Monger XC, Vincent AT. Studying the Association between Antibiotic Resistance Genes and Insertion Sequences in Metagenomes: Challenges and Pitfalls. Antibiotics. 2023; 12(1): 175.
He J, Li C, Cui P, Wang H. Detection of Tn 7-Like Transposons and Antibiotic Resistance in Enterobacterales From Animals Used for Food Production With Identification of Three Novel Transposons Tn 6813, Tn 6814, and Tn 6765. Frontiers in Microbiology. 2020; 11: 2049.
Iyer A, Barbour E, Azhar E, Salabi AAE, Hassan HMA, Qadri I,HarakehS. (2013).Transposable elements in Escherichia coli antimicrobial resistance. Advances in Bioscience and Biotechnology. 2013; 4(03): 415-23.
Laxminarayan R, Duse A, Wattal C, Zaidi AK, Wertheim HF, Sumpradit N, Cars O. Antibiotic resistance—the need for global solutions. The Lancet infectious diseases. 2013; 13(12): 1057-1098.
Lipszyc A, Szuplewska M, Bartosik D. How Do Transposable Elements ActivateExpression of Transcriptionally Silent Antibiotic Resistance Genes? International Journal of Molecular Sciences. 2022; 23(15): 8063.
Mahillon J. Transposons as gene haulers. Apmis. 1998; 106(S84), 29-36.
McEwen SA,Collignon PJ. (2018). Antimicrobial resistance: a one health perspective. Antimicrobial resistance in bacteria from livestock and companion animals. 2018; 521-547.
Mosconi C, Arantes DAC, Gonçalves AS, Alencar RDCG, Oliveira JC, SilvaTA, Batista AC. Immunohistochemical investigations on the expression of programmed cell death ligand 1, human leukocyte antigens G and E, and granzyme B in intraoral mucoepidermoid carcinoma. Archives of Oral Biology. 2017; 83: 55-62.

DOI
https://doi.org/10.62418/ijvph.9.2.2023.63-69
DOWNLOAD FULL ARTICAL
How to cite this article:
Corresponding author’s email ID: drpinkuthakur@gmail.com
Citation: Thakur PK, Parihar R, Pegu SR, Deb R, Niharika J, Das PJ, Sengar GS, Sonowal J, Gupta VK. Identification of Transposable Elements Responsible for AMR in Food Borne Pathogen. Indian Journal of Veterinary Public Health. 2022; 9(2): 63-69.
DOI: https://doi.org/10.62418/ijvph.9.2.2023.63-69