• Print ISSN: 0976-948X
  • Online ISSN: 3048-5738
  • NAAS Rating: 3.35
Isolation and Characterization of Arcobacter from Foods of Animal Origin and Environmental Samples
Volume 9 | Issue 2 | June 2023
AUTHOR(S)
Dasarathi Praveen Kumar, Lakkineni Venkateswara Rao, Angalakuditi Jagadeesh Babu, Anumolu Vijaya Kumar, Arrabelli Shirisha

ABSTRACT

The present study was undertaken to optimize PCR assay for the detection of Arcobacter spp. And Arcobacter butzleri from foods of animal origin and environmental samples and compare its efficacy with conventional culture methods. Primers derived from 16S rRNA and 23S rRNA genes were used which gave specific amplification products of 181 bp and 686 bp for Arcobacter spp. and Arcobacter butzleri, respectively. Out of 240 samples screened (30 each of chicken, mutton, beef, raw milk, faecal samples of cattle, pig, and poultry, and water) for Arcobacter spp., PCR gave 31 isolates (chicken meat-6, mutton-4, beef-3, raw milk-2, faecal samples of cattle-4, faecal samples of pig-6 and faecal samples of poultry-5, water-1) as positive and culture method gave 21 isolates (chicken meat-4, mutton-3, beef-2, raw milk-1, faecal samples of cattle-3, faecal samples of pig-4 and faecal samples of poultry-4, water-nil) as positive. Out of 31 positive isolates by PCR, 15 (chicken meat-3, mutton-2, beef-2, raw milk-1, faecal samples of cattle-2, faecal samples of pig-2 and faecal samples of poultry-2, water-1) were positive for Arcobacter butzleri. Arcobacter spp.is an important emerging food and water-borne pathogens having public health concerns. Traditional methods for detecting and identifying Arcobacter spp. by culture methods are intensive and time-consuming, requiring 5-7 days to get a confirmed positive result. Hence there is a need to develop reliable and rapid methods for the detection of Arcobacter spp. from foods of animal origin.

Aydin F, Gumussoy KS, Atabay HI, Ica T, Abay S. Prevalence and distribution of Arcobacter species in various sources in Turkey and molecular analysis of isolated strains by PCR. Journal of Applied Microbiology. 2007; 103(1): 27-35.
Bagalakote PS, Rathore RS, Ramees TP, Mohan HV, Sumankumar M, Upadhyaya P, Kumar A. Study on prevalence of Arcobacter spp. in human and foods of animal origin. Journal of Veterinary Public Health. 2013; 11: 105-9.
De Oliveira SJ, Baetz AL, Wesley IV, Harmon KM. Classification of Arcobacter species isolated from aborted pig fetuses and sows with reproductive problems in Brazil. Veterinary Microbiology. 1997; 57(4): 347-54.
Ertas N, Dogruer Y, Gonulalan Z, Guner A, Ulger I. Prevalence of Arcobacter species in drinking water, spring water, and raw milk as determined by multiplex PCR. Journal of Food Protection. 2010; 73 (11): 2099-102.
Fera MT, La Camera E, Carbone M, Malara D, Pennisi MG. Pet cats as carriers of Arcobacter spp. in Southern Italy. Journal of Applied Microbiology. 2009; 106(5): 1661-6.
Fernandez H, Villanueva MP, Mansilla I, Gonzalez M, Latif F. Arcobacter butzleri and A. cryaerophilus in human, animals and food sources, in southern Chile. Brazilian Journal of Microbiology. 2015; 46(1): 145-7.
Gugliandolo C, Irrera GP, Lentini V, Maugeri TL. Pathogenic Vibrio, Aeromonas and Arcobacter spp. associated with copepods in the Straits of Messina (Italy). Marine Pollution Bulletin. 2008; 56(3): 600-6.
Harmon KM, Wesley IV. “Identification of Arcobacter isolates by PCR.” Letters in Applied Microbiology. 1996; 23(4): 241-4.
Kabeya H, Maruyama S, Morita Y, Ohsuga T, Ozawa S, Kobayashi Y, Mikami T. Prevalence of Arcobacter species in retail meats and antimicrobial susceptibility of the isolates in Japan. International Journal of Food Microbiology. 2004; 90(3): 303-8.
Lappi V, Archer JR, Cebelinski E, Leano F, Besser JM, Klos RF, Davis JP. An outbreak of foodborne illness among attendees of a wedding reception in Wisconsin likely caused by Arcobacter butzleri. FoodbornePathogens and Disease. 2013; 10(3): 250-5.
Lee MH, Choi C. Survival of Arcobacter butzleri in Apple and Pear Purees. Journal of Food Safety. 2013; 33(3): 333-9.
Miller WG, Wesley IV, On SL, Houf K, Mégraud F, Wang G, Mason CJ. First multi-locus sequence typing scheme for Arcobacter spp. BMC Microbiology. 2009; 9(1): 196.
Mohan HV, Rathore RS, Dhama K, Ramees TP, Patyal A, Bagalkot PS, Kumar A. Prevalence of Arcobacter spp. in humans, animals and foods of animal origin in India based on cultural isolation, antibiogram, PCR and multiplex PCR detection. Asian Journal of Animaland Veterinary Advances. 2014; 9(8): 452-66.
Patyal A, Rathore RS, Mohan HV, Dhama K, Kumar A. Prevalence of Arcobacter spp. in humans, animals and foods of animal origin including seafood from India. Transboundary and Emerging Diseases. 2011; 58(5): 402-10.
Pianta C, Passos DT, Hepp D, Oliveira SJD. Isolation of Arcobacter spp from the milk of dairy cows in Brazil. Ciência Rural. 2007; 37(1): 171-4.
Ramees TP, Rathore RS, Bagalkot PS, Mohan HV, Kumar A, Dhama K.Detection of Arcobacter butzleri and Arcobacter cryaerophilus in clinical samples of humans and foods of animal origin by cultural and multiplex PCR based methods. Asian Journal of Animal and Veterinary Advances. 2014; (9): 243-52.
Rivas L, Fegan N, Vanderlinde P. Isolation and characterization of Arcobacter butzleri from meat. International Journal of Food Microbiology. 2004; 91: 31-41.
Scullion R, Harrington CS, Madden AH. Prevalence of Arcobacter spp. in raw milk and retail raw meats in Northern Ireland. Journal of Food Protection. 2006; 69(8): 1986-90.
Shah AH, ShahMG, Khan MS, Nizamani ZA, Mirani AH, Soomro AH, Zunita Z. Occurrence of novel alleles and sequence types in Arcobacter butzleri. Veterinary Research. 2014; 76.
Van Driessche E, HoufK, Van Hoof J, De Zutter L, Vandamme P. Isolation of Arcobacter species from animal faeces. FEMS Microbiology Letters. 2003; 229(2): 243-8.

DOI
https://doi.org/10.62418/ijvph.9.2.2023.20-26
DOWNLOAD
How to cite this article:
Corresponding author’s email ID: shirisharao54@gmail.com
Citation: Kumar DP, Rao LV, Babu AJ, Kumar AV, Shirisha A. Isolation and Characterization of Arcobacter from Foods of Animal Origin and Environmental Samples. Indian Journal of Veterinary Public Health. 2023; 9(2): 20-26.
DOI: https://doi.org/10.62418/ijvph.9.2.2023.20-26